Please join us at the Sound Bar in Fort Collins from 5 to 7 pm for the CBMSS Fall Meeting! Dr. Sam Myers from the La Jolla Institute for Immunology will be giving a talk about his research which is described below in a short abstract.
We hope to see you all there!
Resolving dynamic OGT-protein interactions reveals its use of adaptor proteins to control cell state transitions
The sole O-GlcNAc transferase, OGT, interacts with nearly 1,000 proteins and glycosylates thousands of proteins in a site-specific manner. How OGT targets its substrates is poorly understood, and the role of the resultant glycosylation events are largely uncharacterized. Here, we demonstrate that OGT uses specific proteins and/or protein complexes to target downstream substrates, which in turn, controls some of the earliest decisions in the developing zygote. We will also discuss technical challenges to study dynamic protein-protein interactions in cells with differing background proteomes, and provide several solutions to better classify false positives and rescue false negatives.
